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KMID : 1123920120260040483
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 4 p.483 ~ p.490
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Effects of Polygoni Cuspidati Radix on the H2O2-treated LLC-PK1 Cell’s Redox Status and NF-ĸB Signaling
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Kim Sol-Ri
Jung Ji-Chun
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Abstract
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This study was designed to identify the effects of Polygoni cuspidati Radix(PCR) on the generation of superoxide anion radicals (?O2 -), nitric oxide (NO), peroxynitrite (ONOO-) in the renal epithelial cells of mouse(LLC-PK1). The effects of PCR on the expression of inflammation-related proteins, IKK-α, phospho-IκB-α, NF-κB (p50, p65), COX-2, iNOS, IL-1β, VCAM-1, were examined by western blotting. For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 2’,7’-dichloro dihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) were used. Protein expression levels of IKK-α, phospho-IκB-α, NF-κB (p50, p65), COX-2, iNOS, IL-1β, VCAM-1 were assayed by western blot. PCR reduced H2O2-induced cell death dose-dependently. It inhibited the generation of ?O2 -, NO, ONOO- and PGE2 in the H2O2-treated LLC-PK1 cells in vitro. PCR inhibited the espression of IKK-α, phospho-IκB- α, COX-2, iNOS, IL-1β and VCAM-1 genes by means of decreasing the NF-κB activation. These results suggest that PCR is an effective NO, ?O2
-, ONOO- scavenger, and this substance recommended to be applied in treatment for the inflammatory process and inflammation-related disease.
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KEYWORD
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Polygoni cuspidati Radix, LLC-PK1 cell, H2O2, Reactive oxygen species, NF-κB activation
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